Generation of human induced pluripotent stem cells in defined, feeder-free conditions
نویسندگان
چکیده
منابع مشابه
Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions.
Recently, iPSCs have attracted attention as a new source of cells for regenerative therapies. Although the initial method for generating iPSCs relied on dermal fibroblasts obtained by invasive biopsy and retroviral genomic insertion of transgenes, there have been many efforts to avoid these disadvantages. Human peripheral T cells are a unique cell source for generating iPSCs. iPSCs derived from...
متن کاملGeneration of Induced Pluripotent Stem Cells from Human Amniotic Fluid Cells by Reprogramming with Two Factors in Feeder-free Conditions
The ectopic expression of transcription factors for reprogramming human somatic cells to a pluripotent state represents a valuable resource for the development of in vitro-based models for human disease and has great potential in regenerative therapies. However, the majority of studies have used skin fibroblasts to generate induced pluripotent stem cells (iPSCs) that typically require the enfor...
متن کاملdifferentiation of definitive endoderm from human induced pluripotent stem cells on hmscs feeder in a defined medium
background: the definitive endoderm (de) differentiation using the undefined media and non-human feeders can cause contaminations in the generated cells for therapeutic applications. therefore, generating safer and more appropriate de cells is needed. this study compared five different methods to establish an appropriate method for inducing an efficient de differentiation from human induced plu...
متن کاملFeeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells.
Ectopic expression of transcription factors can reprogram somatic cells to a pluripotent state. However, most of the studies used skin fibroblasts as the starting population for reprogramming, which usually take weeks for expansion from a single biopsy. We show here that induced pluripotent stem (iPS) cells can be generated from adult human adipose stem cells (hASCs) freshly isolated from patie...
متن کاملA New Feeder-Free Technique to Expand Human Embryonic Stem Cells and Induced Pluripotent Stem Cells
The optimal maintenance of human embryonic stem cells (hESC) in vitro is generally observed in the presence of a feeder-layer of mouse embryonic fibroblasts in a serum-containing medium. Various approaches are now available to remove the feeder requirement. Today, the best feeder-free system for the maintenance of hESC and induced pluripotent stem (iPS) cells is based on a serum-replacement med...
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ژورنال
عنوان ژورنال: Stem Cell Research
سال: 2016
ISSN: 1873-5061
DOI: 10.1016/j.scr.2016.05.006